INS No. 160e
CAS No. 1107-26-2
CI Food Orange 6
CI No. 40820
β-Apo-8′-carotenal is an aldehydic carotenoid that is widely distributed in nature. It consists of deep violet crystals with metallic luster or crystalline powder, and can be isolated from spinach, oranges, grass, tangerines, and marigolds.
β-apo-8′-carotenal can be used to color confectionery, bakery products, snack food, juices, fruit drinks, soups, jams, jellies, gelatins, processed cheese, margarine, salad dressings, fats and oils.
β-apo-8′-carotenal (INS No 160e) is added to foods and beverages at concentrations up to a maximum permitted level (MPL) as established by the Codex Alimentarius Commission and published in the General Standard of Food Additives (GSFA).
No relevant genotoxicity data were identified. Ethyl and methyl β-apo-8′-carotenate, free and esterified vitamin A, and vitamin A alcohol were identified as metabolites of β-apo-8′-carotenal. The low acute oral toxicity of β-apo-8’-carotenal is demonstrated by a mouse LD50 that exceeds 10 g/kg bw. Apart from the pigment deposition in the liver and kidneys, no adverse effects occurred in rats fed up to 500 mg/kg bw/day β-apo-8′-carotenal for 34 weeks. No adverse effects were reported in a summary of multigeneration studies where rats were fed 250 mg/kg bw/day of β-apo-8’-carotenal or 500 mg/kg bw/day of its methyl ester. The fertility of rats fed 500 mg/kg bw/day of β-apo-8’-carotenal or its ethyl ester was also unaffected.
Evaluation of certain food additives (Eighty-seventh report of the Joint FAO/WHO Expert Committee on Food Additives) WHO Technical Report Series, No. 1020, 2019. Available online
JECFA (1974). Toxicological evaluation of some food colors, enzymes, flavor enhancers, thickening agents and certain food additives. FAO Nutr. Mtgs. Ser. No. 54; WHO Tech. Rept. Ser. No. 557. Eighteenth meeting of the Joint FAO/WHO Expert Committee on Food Additives. Available online
Evaluation of certain food additives and contaminants: 74th report of the JECFA. WHO Technical Report Series No. 966. Available online
EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS); Scientific Opinion on the re-evaluation of β-apo-8’-carotenal (E 160e) as a food additive. EFSA Journal 2012;10(3):2499. [46 pp.] Available online