INS No. 160a(i); Carotenes, beta-, synthetic
INS No. 160a(ii); Carotenes, beta-, vegetable
INS No. 160a(iii); Carotenes, beta-, Blakeslea trispora
INS No. 160(iv); beta-carotene-rich extract from Dunaliella salina
E160a(ii); plant carotenes
E160a(iii); beta-carotene from Blakeslea trispora
E160a(iv); algal carotenes
β- Carotene is prepared synthetically or obtained from natural sources. Synthetic β- Carotene occurs as red crystals or as crystalline powder. It consists predominantly of all-trans-β-carotene, but may also contain minor amounts of cis-isomers and other carotenoids such as all-trans-retinal, beta-apo-12′-carotenal, and beta-apo-10′-carotenal.
Carotenes (vegetable) are obtained by solvent extraction of carrots (Daucus carota), oil of palm fruit (Elaeis guinensis), sweet potato (Ipomoea batatas) and other edible plants with subsequent purification. The main coloring principles are alpha-and β-Carotenes of which β-Carotene account for the major part. Carrot oil is the liquid or solid portion of the mixture or the mixture itself obtained by the hexane extraction of edible carrots.
β-Carotene from Blakeslea trispora occurs as red, brownish-red, or purple-violet crystals or as crystalline powder, with the color varying depending on the solvents used and the crystallization conditions. β-Carotene from Blakeslea trispora consists predominantly of all-trans-β-carotene, but may also contain minor amounts of cis-isomers and other carotenoids, of which γ-carotene is the most characteristic type.
β-Carotene-Rich Extract from Dunaliella salina is obtained by extraction from strains of the algae Dunaliella salina using the essential oil d-limonene. The extract is then prepared as a suspension in vegetable oil after removal of the essential oil. The main coloring principles are trans- and cis–isomers of ß-carotene together with minor amounts of other carotenes including α-carotene, lutein, zeaxanthin and cryptoxanthin.
β –carotene can be used in a wide range of food and beverages including cider, malt beverages, water-based flavored drinks, margarines, cheeses, cake fillings, custards, yogurts, processed nuts, precooked pastas and noodles.
Carotenoids, including beta-Carotenes, Blakeslea trispora (INS No. 160a(iii) and beta-Carotenes, synthetic (INS No. 160a(i)) are added to foods and beverages at concentrations in more than 70 food categories up to a maximum permitted level (MPL) as established by the Codex Alimentarius Commission and adopted in the General Standard of Food Additives. Additionally, vegetable beta-Carotenes (INS No. 160a(ii)) are added to foods and beverages at concentrations in more than 80 food categories up to a MPL as adopted in the General Standard of Food Additives. Many applications for the use of beta-Carotene-rich extract from Dunaliella Salina (INS No. 160a(iv)) as a color additive in foods and beverages have been proposed and are pending adoption.
Evaluation of certain food additives (Eighty-seventh report of the Joint FAO/WHO Expert Committee on Food Additives) WHO Technical Report Series, No. 1020, 2019. Available online
Evaluation of certain food additives: prepared by the Eighty-fourth meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA). Geneva: World Health Organization and Food and Agriculture Organization of the United Nations; 2019 (WHO Food Additives Series, No. 75). Available online
JECFA (1993) Evaluation of certain food additives and contaminants. 41st report of the Joint FAO/WHO Expert Committee on Food Additives. WHO Technical Report Series 837. Available online
EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS); Scientific Opinion on the reevaluation of Mixed Carotenes (E 160a (i)) and beta-Carotene (E 160a (ii)) as a food additive. EFSA Journal 2012;10(3):2593. [67 pp.] doi:10.2903/j.efsa.2012.2593. Available online